Diagnosis and phylogenetic analysis of bovine viral diarrhea virus in cattle (Bos taurus) and buffaloes (Bubalus bubalis) from the Amazon region and Southeast Brazil

ABSTRACT: Bovine viral diarrhea virus (BVDV) is a highly infectious pathogen that affects bovines worldwide leading to great economic impact. Although Brazil has the largest commercial cattle population throughout world and an increasing buffalo breeding industry, country no control or eradication program for BVDV. In this perspective, aim of study was evaluate occurrence BVDV in buffaloes from two Brazilian states. Four different ELISA tests were performed confirmed by neutralization testing (VNT). The presence antibodies serum plasma 77 six herds (ELISA-1 ELISA-4) 89 three through detected. Extraction RNA samples detection RT-PCR analysis. Amplified nucleotide sequences used construct phylogenetic tree. cattle, ELISA-1 detected 49.4% seropositive animals, while ELISA-4 37.7%. buffaloes, failed detect any ELISA-2 ELISA-3 20.2% 21.3%. Eight nine tested had animals. rate PCR positive animals 6.5% 9% buffaloes. Subtype 1d found subtypes 1f This first-time subtype been reported Brazil. absence seems be favoring spread herds. addition, improvement diagnostic strategies are required.